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- Title
Photodynamic Therapy of Prostate Cancer by Means of 5-Aminolevulinic Acid-Induced Protoporphyrin IX – In vivo Experiments on the Dunning Rat Tumor Model.
- Authors
Zaak, Dirk; Sroka, Ronald; Stocker, Susanne; Bise, Karl; Lein, Michael; Höppner, Michael; Frimberger, Dominic; Schneede, Peter; Reich, Oliver; Kriegmair, Martin; Knüchel, Ruth; Baumgartner, Reinhold; Hofstetter, Alfons
- Abstract
Objective: In order to expand the use of photodynamic therapy (PDT) in the treatment of prostate carcinoma (PCA), the aim of this study was to evaluate PDT by means of 5-aminolevulinic acid (5-ALA)-induced protoporphyrin IX (PPIX) in an in vivo tumor model. Methods: The model used was the Dunning R3327 tumor. First of all, the pharmacokinetics and the localization of PPIX were obtained using fluorescence measurement techniques. Thereafter, PDT using 150 mg 5-ALA/kg b.w. i.v. was performed by homogenous irradiation of the photosensitized tumor (diode laser λ = 633 nm). The tumors were resected 2 days post-PDT and the extent of the necrosis was determined histopathologically. Results: The kinetics of PPIX fluorescence revealed a maximum intensity in the tumor tissue within 3 and 4.5 h post-application of 5-ALA. At this time, specific PPIX fluorescence could be localized selectively in the tumor cells. The PDT-induced necrosis (n = 18) was determined to be 94 ± 12% (range 60–100%), while the necrosis of the controls (n = 12) differs significantly (p < 0.01), being less than 10%. Conclusion: These first in vivo results demonstrate the effective potential of 5-ALA-mediated PDT on PCA in an animal model. Copyright © 2004 S. Karger AG, Basel
- Subjects
PHOTOCHEMOTHERAPY; PROSTATE cancer; TUMORS; NECROSIS; PHARMACOKINETICS
- Publication
Urologia Internationalis, 2004, Vol 72, Issue 3, p196
- ISSN
0042-1138
- Publication type
Article
- DOI
10.1159/000077114