We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
In vitro enzymatic assays of photosynthetic bacterial 3-vinyl hydratases for bacteriochlorophyll biosyntheses.
- Authors
Teramura, Misato; Harada, Jiro; Tamiaki, Hitoshi
- Abstract
A chlorosome is a large and efficient light-harvesting antenna system found in some photosynthetic bacteria. This system comprises self-aggregates of bacteriochlorophyll (BChl) c, d, or e possessing a chiral 1-hydroxyethyl group at the 3-position, which plays a key role in the formation of the supramolecule. Biosynthesis of chlorosomal pigments involves stereoselective conversion of 3-vinyl group to 3-(1-hydroxyethyl) group facilitated by a 3-vinyl hydratase. This 3-vinyl hydration also occurs in BChl a biosynthesis, followed by oxidation that introduces an acetyl group at the 3-position. Herein, we present in vitro enzymatic assays of paralogous 3-vinyl hydratases derived from green sulfur bacteria, Chlorobaculum tepidum and Chlorobaculum limnaeum, the filamentous anoxygenic phototroph Chloroflexus aurantiacus, and the chloracidobacterium Chloracidobacterium thermophilum. All the hydratases showed hydration activities. The biosynthetic pathway of BChl a and other chlorosomal pigments is discussed considering the substrate specificity and stereoselectivity of the present hydratases.
- Subjects
CHLOROSOMES; BACTERIOCHLOROPHYLLS; CHLOROPHYLL synthesis; LIGHT-harvesting complex (Photosynthesis); PHOTOSYNTHETIC bacteria; HYDRATASES
- Publication
Photosynthesis Research, 2018, Vol 135, Issue 1-3, p319
- ISSN
0166-8595
- Publication type
Article
- DOI
10.1007/s11120-017-0415-6