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- Title
Highly sensitive fluorescence biosensors for sparfloxacin detection at nanogram level based on electron transfer mechanism of cadmium telluride quantum dots.
- Authors
Liang, Wanjun; Liu, Shaopu; Song, Jing; Hao, Chenxia; Wang, Linlin; Li, Dan; He, Youqiu
- Abstract
A sensitive fluorescence biosensor for determining sparfloxacin (SPF) based on the electron transfer mechanism and the fluorescence quenching effect of SPF to cadmium telluride quantum dots (CdTe QDs) was developed. The mechanism of the interaction between SPF and CdTe QDs was investigated by UV/Vis absorption and fluorescence spectroscopy. The biosensor could be used for the determination of SPF with a high sensitivity. Under optimum conditions, the linear range was from 0.28 to 40 μg SPF ml with a correlation coefficient of 0.9983, and the detection limit (3δ/k) was 83.7 ng SPF ml. Furthermore, this method has been applied to the determination of SPF in the synthetic environmental water samples and the spiked human serum samples with good results.
- Subjects
BIOSENSORS; MACHINE design; OPTICAL properties of cadmium telluride; QUANTUM dots spectra; FLUORESCENCE quenching; OXIDATION-reduction reaction
- Publication
Biotechnology Letters, 2015, Vol 37, Issue 5, p1057
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-015-1767-7