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- Title
Short-Term l-arginine Treatment Mitigates Early Damage of Dermal Collagen Induced by Diabetes.
- Authors
Miler, Irena; Rabasovic, Mihailo D.; Askrabic, Sonja; Stylianou, Andreas; Korac, Bato; Korac, Aleksandra
- Abstract
Changes in the structural properties of the skin due to collagen alterations are an important factor in diabetic skin complications. Using a combination of photonic methods as an optic diagnostic tool, we investigated the structural alteration in rat dermal collagen I in diabetes, and after short-term l-arginine treatment. The multiplex approach shows that in the early phase of diabetes, collagen fibers are partially damaged, resulting in the heterogeneity of fibers, e.g., "patchy patterns" of highly ordered/disordered fibers, while l-arginine treatment counteracts to some extent the conformational changes in collagen-induced by diabetes and mitigates the damage. Raman spectroscopy shows intense collagen conformational changes via amides I and II in diabetes, suggesting that diabetes-induced structural changes in collagen originate predominantly from individual collagen molecules rather than supramolecular structures. There is a clear increase in the amounts of newly synthesized proline and hydroxyproline after treatment with l-arginine, reflecting the changed collagen content. This suggests that it might be useful for treating and stopping collagen damage early on in diabetic skin. Our results demonstrate that l-arginine attenuates the early collagen I alteration caused by diabetes and that it could be used to treat and prevent collagen damage in diabetic skin at a very early stage.
- Subjects
ARGININE; COLLAGEN; DIABETES; ETIOLOGY of diabetes; RAMAN spectroscopy; AMIDES; HYDROXYPROLINE
- Publication
Bioengineering (Basel), 2024, Vol 11, Issue 4, p407
- ISSN
2306-5354
- Publication type
Article
- DOI
10.3390/bioengineering11040407