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- Title
Medical Grade Honey as a Promising Treatment to Improve Ovarian Tissue Transplantation.
- Authors
Azevedo, Ana Rita; Pais, Ana Sofia; Almeida-Santos, Teresa; Pires, Virgínia M. R.; Pessa, Pedro; Marques, Carla C.; Nolasco, Sofia; Castelo-Branco, Pedro; Prates, José A. M.; Lopes-da-Costa, Luís; Laranjo, Mafalda; Botelho, Maria Filomena; Pereira, Rosa M. L. N.; Pimenta, Jorge M. B. G. A.
- Abstract
Ovarian tissue cryopreservation is a female fertility preservation technique that presents major challenges for the maintenance of follicular viability after transplantation. The aim of this study was to evaluate and compare the application of L-Mesitran Soft®, a product containing 40% medical grade honey (MGH), with other strategies to improve ovarian grafts' viability. For this purpose, bovine ovarian tissue was vitrified, warmed and randomly assigned to culture groups: (1) control, (2) MGH 0.2% in vitro, (3) MGH in vivo (direct application in the xenotransplantation), (4) vascular endothelial growth factor (VEGF 50 ng/mL) and (5) vitamin D (100 Nm), during a 48 h period. A sixth group (6) of fragments was thawed on transplantation day and was not cultured. The tissue was xenotransplanted into immunodeficient (Rowett nude homozygous) ovariectomized rats. Grafts were analyzed 48 h after culture, and 7 and 28 days after transplantation. The tissue was subjected to histological and immunohistochemical analysis. Treatments using MGH showed the highest angiogenic and cell proliferation stimulation, with cellular apoptosis, within a healthy cellular turnover pathway. In conclusion, MGH should be considered as a potentially effective and less expensive strategy to improve ovarian tissue transplantation.
- Subjects
OVARIAN transplantation; TRANSPLANTATION of organs, tissues, etc.; VASCULAR endothelial growth factors; HONEY; FERTILITY preservation
- Publication
Bioengineering (Basel), 2022, Vol 9, Issue 8, pN.PAG
- ISSN
2306-5354
- Publication type
Article
- DOI
10.3390/bioengineering9080357