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- Title
CfNop12 参与调控果生刺盘孢生长发育、低温胁 迫响应和致病力.
- Authors
姚权; 李司政; 王成玉; 李河
- Abstract
Colletotrichum fructicola is a major causal pathogen of oil-tea tree. The purpose of this study is to investigate the biological function of the RNA-binding proteins CfNop12 of C. fructicola and provide a theoretical basis for the prevention and control of oil-tea tree anthracnose. Based on the principle of homologous recombination, the CfNOP12 gene knockout vector fragment was constructed and transformed into the protoplast of C. fructicola to get the mutant strain of ΔCfnop12. The mutant strain was verified by PCR amplification. The PCR-amplified CfNOP12 gene-containing complement of the promoter was taken to construct a complementary vector fragment which was transformed into the mutant protoplasts. The complemented strain was screened by fluorescence. The biological phenotypes of wild-type strain, the ΔCfnop12 mutant and the gene complementation ΔCfnop12-C were measured. The results showed that the vegetative growth, conidiation, and appressorium formation rate of ΔCfnop12 was significantly lower than those of the wild-type strain and the complemented strain. Compared with the wild-type strain, the CfNOP12 gene deletion mutant was more sensitive to osmotic stress and cell wall-damaging agents. At the low temperature, the growth rate of the mutant was markedly slowed down. In addition, the distribution of chitin of the mutant was not aggregated at the tip of mycelium. Taken together, our investigation reveals that the RNA-binding protein CfNop12 plays a critical role in development, cold stress response and pathogenicity of C. fructicola.
- Subjects
COLLETOTRICHUM; GENES
- Publication
Mycosystema, 2023, Vol 42, Issue 11, p2257
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.230089