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- Title
液泡分选蛋白CfVps17 参与调控果生刺盘孢的生 长发育和致病力.
- Authors
李玲玲; 张盛培; 李河
- Abstract
Colletotrichum fructicola is a major pathogen causing anthracnose on Camellia oleifera. This experiment aims at revealing the biological functions of the putative vacuolar sorting protein CfVps17, providing a basis for illuminating the pathogenic molecular mechanism of C. fructicola regulated by this protein. The over-lap method was used to construct the CfVPS17 gene-deleted fragment which was transformed into the protoplasts of wild-type strain CFLH16 by PEG-mediated method for obtaining mutant strain ΔCfvps17 through validation screening. A complement vector pYF11::CfVPS17 of the CfVPS17 gene was constructed and transformed into the mutant strain’s protoplast by PEG-mediated method. The complementary strain ΔCfvps17-C was obtained by fluorescence screening. The biological phenotypes of the wild-type strain CFLH16, the mutant strain ΔCfvps17, and the complementary strain ΔCfvps17-C including vegetative growth, appressorium formation, stress response, accumulation of glycogen, and pathogenicity were further tested. The result indicated that the mycelial growth rate, conidiation and appressorium formation rate of the ΔCfvps17 were significantly reduced, glycogen storage in mycelium declined, and tolerance to Congo red and NaCl increased, in comparison with CFLH16 and ΔCfvps17-C. Pathogenicity test showed that the ΔCfvps17 lost the ability to infect Ca. oleifera leaves. Facts prove that the vacuoler sorting protein CfVps17 is involved in regulating the growth, conidiation, appressorium formation, response to stress, accumulation of glycogen and pathogenicity of C. fructicola.
- Subjects
CONGO red (Staining dye); CAMELLIA oleifera; GLYCOGEN; PHENOTYPES; SALT
- Publication
Mycosystema, 2021, Vol 40, Issue 8, p2102
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.210051