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- Title
果生刺盘孢CfHAC1 调控应答二硫苏糖醇胁迫的转录组分析.
- Authors
李司政; 李河
- Abstract
Colletotrichum fructicola is a major pathogen causing anthracnose on Camellia oleifera. In previous study, we characterized that the bZIP transcription factor CfHac1 controlled the development and pathogenicity of C. fructicola. To identify the role of CfHac1 in the endoplasmic reticulum (ER) stress in C. fructicola, the growth of a ΔCfhac1 mutant strain of C. fructicola was determined on the PDA medium containing dithiothreitol, an ER stress factor. The results revealed that the ΔCfhac1 strain was sensitive to dithiothreitol. To uncover the potential mechanisms of CfHac1 contributions to pathogenicity and response to ER stress, the transcriptome profiles of the wild type strain and mutant ΔCfhac1 of C. fructicola were characterized with high-throughput sequencing technology. The data indicated that, relative to the wild type, there were 2 680 differentially expressed genes (DEGs) in mutant ΔCfhac1, of which 1 181 were up-regulated and 1 499 were down-regulated. The Gene Ontology classification showed that the DEGs were mainly related to catalytic activity, binding, metabolic process, cellular process, cellular component organization or biogenesis, regulation of biological process and response to stimulus pathway. The KEGG pathway analysis also revealed that the up-regulated expressed genes were mainly related to ribosome, ribosome biogenesis in eukaryotes, RNA transport and cyanoamino acid metabolism pathways; the down-regulated expressed genes were mainly related to protein processing in ER, N-glycan biosynthesis, steroid biosynthesis, and protein export pathways. Genes involved in ER stress response and virulence were down-regulated at transcriptional level in the ΔCfhac1 strain. This study provides useful data concerning the effect of CfHac1 in endoplasmic reticulum stress response at the genome level and a basis for illuminating pathogenic mechanism of C. fructicola.
- Subjects
ORGANELLE formation; CAMELLIA oleifera; ENDOPLASMIC reticulum; CELL anatomy; TRANSCRIPTION factors; RIBOSOMES
- Publication
Mycosystema, 2020, Vol 39, Issue 10, p1886
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.200081