We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
抗产气荚膜梭菌感染的鸡源益生菌筛选及评估.
- Authors
杨明珠; 张美红; 文 秋; 胡永飞; 刘 丹
- Abstract
This study aims to screen out the chicken probiotic strains with excellent inhibition of Clostridium perfringens in vivo, and evaluate the effect on intestinal inflammation on Arbor Acres (AA) broilers infected with Clostridium perfringens. In this study, strains with inhibitory activity of Clostridium perfringens were screened by bacteriostatic zone method, and trains with the best performance was selected by evaluating the growth curve, acid resistance, bile resistance, enzyme production activity and hemolytic characteristics. A total of 210 1-day-old AA male broilers were randomly divided into three treatment groups with seven replicates per treatment and 10 broilers per replicate, which were control group, Clos⁃ tridium perfringens-infected group, and Enterococcus faecalis group (4.0×109 CFU/kg) in animal trial. The trial lasted for 40 days. The results showed as follows: Enterococcus faecalis A14S have excellent ability of acid and bile resistance enzyme productivity, its supernatant could significantly inhibit the formation of Clostridium perfringens biofilm (P<0.05) and the hemolysis was γ type. The results of animal experiments showed that compared with Clostridium per⁃ fringens- infected group, dietary supplementation of Enterococcus faecalis A14S 4.0×109 CFU/kg im⁃ proved the decrease of thymus organ index caused by Clostridium perfringens challenge (P<0.05), it also significantly increased the ratio of intestinal villus height to crypt depth (P<0.05). The supplementation of Enterococcus faecalis A14S had the tendency of decrease the concentration of D-lactate (DLA) in serum (P<0.10); it significantly decreased the mRNA expression of pro-inflammatory cytokines interferon-gamma (IFN-γ) and toll-like receptor 4 (TLR4), tended to decrease inducible nitric oxide synthase (iNOS). Taken together, Enterococcus faecalis A14S was shortlisted with a good inhibitory effect on Clostridium perfringens, dietary supplementation of Enterococcus faecalis A14S at 4.0×109 CFU/kg could maintained gut health by boost gut barrier and alleviate the level of the inflammatory response caused by Clostridium perfringens infection.
- Publication
Feed Industry, 2023, Vol 44, Issue 13, p99
- ISSN
1001-991X
- Publication type
Article
- DOI
10.13302/j.cnki.fi.2023.13.014