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- Title
创伤后应激障碍大鼠海马与前额叶皮质中OREXIN受体的失调.
- Authors
刘燕; 高俊巧; 李峰; 周芹; 宋月晗
- Abstract
Objective: To study the changes of serum corticosterone and neuron-specific enolase in rats with post-traumatic stress disorder, as well as disorders of orexin (Orexin, ORX) receptors in cerebrospinal fluid, hippocampus and prefrontal cortex. Method: The rat model of PTSD was established by single prolonged stimulation (SPS). Analysis of corticosterone and neuron-specific enolase in rat serum and changes of Orexin receptor in cerebrospinal fluid by enzyme-linked immunosorbent assay (Elisa). The expression of orexin receptor gene in the two brain regions was detected by real-time fluorescence quantitative polymerase chain reaction (RT-PCR). Results: The contents of corticosterone and neuron-specific enolase in serum were significantly increased (P<0.01). The levels of Orexin A and Orexin B in the cerebrospinal fluid of the model group were increased, which was significantly different from the normal group(P<0.01). And the ORX1R and ORX2R in the hippocampus and cortex of the model group were significantly decreased, which was significantly different from the normal group (P<0.01). Conclusion: PTSD resulted in the increase of corticosterone and neuron-specific enolase in serum, and its mechanismmay be related to the level of orexin receptor.
- Subjects
CEREBROSPINAL fluid; ENZYME-linked immunosorbent assay; POST-traumatic stress disorder; PREFRONTAL cortex; ENOLASE
- Publication
Progress in Modern Biomedicine, 2020, Vol 20, Issue 9, p1406
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2020.08.002