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- Title
两种耐热磷酸化酶的构建及高效催化合成红景天苷.
- Authors
何聪芬; 黄爱清; 王巧娥; 张贵友; 余磊; 王洪钟
- Abstract
Objective: Thermostable cellobiose phosphorylase and sucrose phosphorylase were produced by recombinant strain E.coli BL21/pET-Spase and E.coli BL21/pET-Cpase. By combined use of these two phosphorylases, salidroside was efficiently synthesized from inexpensive sucrose. Methods: First, thermostable cellobiose phosphorylase and sucrose phosphorylase recombinant strains were constructed. Then two phosphorylases with sucrose, tyrosol and phosphoric acid were combined. Finally, the mixture was reacted and produced salidroside. Results: At optimal conditions, 23.7 g/L of salidroside was formed over 32 h from 1200 U/L thermostable sucrose phosphorylase, 500 U/L thermostable cellobiose phosphorylase, 110 g/L sucrose, 30 g/L Tyrosol and 50 m M phosphoric acid at the pH7.0 and 45 °C. Conclusion: In this study, thermostable cellobiose phosphorylase and sucrose phosphorylase were employed as the catalyst, and salidroside was successfully synthesized in high yield. And the thermostable phosphorylases constructed in this study had high activity, which offers a new way to expand the ability of phosphorylases in preparation of β-D-glucosides.
- Publication
Progress in Modern Biomedicine, 2019, Vol 19, Issue 10, p1801
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2019.10.001