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- Title
Smad7 对肝癌细胞增殖和迁移的影响及其临床意义.
- Authors
王玉林; 董菁; 王琳; 高雪辰; 沈怡敏
- Abstract
Objective: To investigate the role of Smad7 in the Hepatocellular carcinoma (HCC) migration and proliferation and its clinical significance. Methods: Through transfecting pcDNA3.1 (+)-Smad7 or siRNA Smad7 to overexpress or knockdown the Smad7 expression in HCC cell lines HepG2 and Huh7. The MTT assays were used to test the role of Smad7 in proliferation of HCC cells. Transwell and wound-healing assays were used to detect the effect of Smad7 on migratory ability in both tow cell lines. RT-PCR was used to test the Smad7 expression in 9 clinical HCC patients' specimens. Results: As the results, overexpression of Smad7 significantly inhibited the proliferation of cells compared with the control group, while knockdown Smad7 promoted the proliferation. At the same time, overexpression of Smad7 could inhibit the migratory ability of HCC cells compared with the control group, while knockdown smad7 could accelerate this ability. The expression of Smad7 in cancer tissue was significantly lower compared with normal mucosa tissue adjacent to cancer. Conclusions: Smad7 is a kind of anti-progressive molecule in HCC.
- Publication
Progress in Modern Biomedicine, 2017, Vol 17, Issue 23, p4437
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2017.23.008