We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Membrane-Bound NAC Transcription Factor ANAC013 Functions in Mitochondrial Retrograde Regulation of the Oxidative Stress Response in Arabidopsis.
- Authors
Clercq, Inge De; Vermeirssen, Vanessa; Aken, Olivier Van; Vandepoele, Klaas; Murcha, Monika W.; Law, Simon R.; Inzé, Annelies; Ng, Sophia; Ivanova, Aneta; Rombaut, Debbie; van de Cotte, Brigitte; Jaspers, Pinja; Peer, Yves Van de; Kangasjärvi, Jaakko; Whelan, James; Breusegem, Frank Van
- Abstract
Upon disturbance of their function by stress, mitochondria can signal to the nucleus to steer the expression of responsive genes. This mitochondria-to-nucleus communication is often referred to as mitochondrial retrograde regulation (MRR). Although reactive oxygen species and calcium are likely candidate signaling molecules for MRR , the protein signaling components in plants remain largely unknown. Through meta-analysis of transcriptome data, we detected a set of genes that are common and robust targets of MRR and used them as a bait to identify its transcriptional regulators. In the upstream regions of these mitochondrial dysfunction stimulon (MDS) genes, we found a cis -regulatory element, the mitochondrial dysfunction motif (MDM), which is necessary and sufficient for gene expression under various mitochondrial perturbation conditions. Yeast one-hybrid analysis and electrophoretic mobility shift assays revealed that the transmembrane domain–containing NO APICAL MERISTEM/ ARABIDOPSIS TRANSCRIPTION ACTIVATION FACTOR/CUP-SHAPED COTYLEDON transcription factors (ANAC013, ANAC016, ANAC017, ANAC053, and ANAC078) bound to the MDM cis -regulatory element. We demonstrate that ANAC013 mediates MRR -induced expression of the MDS genes by direct interaction with the MDM cis -regulatory element and triggers increased oxidative stress tolerance. In conclusion, we characterized ANAC013 as a regulator of MRR upon stress in Arabidopsis thaliana.
- Subjects
TRANSCRIPTION factors; OXIDATIVE stress; COTYLEDONS; MITOCHONDRIA; ARABIDOPSIS; REACTIVE oxygen species
- Publication
Plant Cell, 2013, Vol 25, Issue 9, p3472
- ISSN
1040-4651
- Publication type
Article
- DOI
10.1105/tpc.113.117168