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- Title
板栗果实内生真菌群落组成及分化特征.
- Authors
闾 星; 窦桂铭; 汪慧华; 李如华; 江泽平; 李志朋; 严东辉
- Abstract
[Objective] Exploring the composition and diversity of the fruit endophytic fungi community is the basis for understanding the functions and mechanisms of fruit healthy microbiome. [Method] The diversity and composition of endophytic fungal communities in healthy fruit and its kernel (cotyledon) and shell (including seedcoat) parts of six chestnut cultivars were analyzed using high-throughput sequencing technology and traditional tissue isolation methods. [Result] A total of 3,229,205 high-quality sequences were generated from 36 samples of fruits and 2 tissues of six cultivars, and 1,557 fungal OTUs were annotated, belonging to 10 phyla, 38 classes, 97 orders, 203 families, and 350 genera. In chestnut fruit, Ascomycota (average relative abundance 80.40%) had the highest abundance, followed by Basidiomycota (average relative abundance 7.70%), and the dominant class were Sordariomycetes (average relative abundance 43.00%) and Saccharomycetes (average relative abundance 15.10%). The shared fungal taxa among species was enriched in Dothideomycetes with abundance >90%. Among chestnut fruit varieties, Ascomycota was also the most abundant group in each variety, with a relative abundance of 57.96%~90.15%, followed by Basidiomycota(3.28%~23.71%); At the class level, the dominant groups changed. The relative abundance of Saccharomycetes fungi (39.21%) was the highest in Maobanhong varieties, while Sordariomycetes fungi were the most abundant group (29.47%~59.56%) among the other five chestnut cultivars. Among the fruit tissues, the kernel tissue had higher unique fungal diversities than the shell, and the unique dominant groups of kernel tissue were all Basidiomycetes, especially the Russulales of Tremellomycetes. LEfSe analysis showed that there were significant differences in community composition or populations in the shell and kernel tissue, with Fusarium, Metschnikowia, Meyerozyma, and Debaryomyces fungi having significant dominance in kernel tissue. A total of 2346 effective strains were obtained by artificial culturable method, which belonged to Ascomycetes, Basidiomycetes and Mucoromycota. Except for yeasts that need special medium to be screened, the dominant fungal groups on fruits and tissues obtained by artificial culturing methods were similar to the results of high-throughput sequencing technology in terms of community composition and abundance. [Conclusion] Chestnut fruit has abundant endophytic fungal community composition and population diversity dominated by Sordariomycetes and Saccharomycetes, but the community-building species are mainly from Dothieomycetes. The endophytic fungal community composition is significantly different among chestnut fruit cultivars. Chestnut kernel has a richer diversity of exclusive endophytic fungi than shell part. There is a obvious differentiation on the composition of endophytic fungal community between chestnut kernel and shell tissue. This paper is the first systematic report on the compositional structure of endophytic fungi microbiota in chestnut fruit, which provides a basis for using chestnut healthy microbiome to prevent and control fruit diseases and to understand the nut fruit microbiome.
- Subjects
ENDOPHYTIC fungi; CULTIVARS; FRUIT composition; NUCLEOTIDE sequencing; FUNGAL communities; CASTANEA; BASIDIOMYCETES; NUTS
- Publication
Forest Research, 2022, Vol 35, Issue 4, p9
- ISSN
1001-1498
- Publication type
Article
- DOI
10.13275/j.cnki.lykxyj.2022.004.002