We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Differentiation Induction of Mesenchymal Stem Cells by a Au Delivery Platform.
- Authors
Yang, Meng-Yin; Chiu, Cheng-Di; Ke, Yi-Chun; Yang, Yi-Chin; Chang, Kai-Bo; Chen, Chien-Min; Lee, Hsu-Tung; Tang, Chien-Lun; Liu, Bai-Shuan; Hung, Huey-Shan
- Abstract
Au decorated with type I collagen (Col) was used as a core material to cross-link with stromal cell-derived factor 1α (SDF1α) in order to investigate biological performance. The Au-based nanoparticles were subjected to physicochemical determination using scanning electron microscopy (SEM), dynamic light scattering (DLS) and ultraviolet–visible (UV-Vis) and Fourier-transform infrared spectroscopy (FTIR). Mesenchymal stem cells (MSCs) were used to evaluate the biocompatibility of this nanoparticle using the MTT assay and measuring reactive oxygen species (ROS) production. Also, the biological effects of the SDF-1α-conjugated nanoparticles (Au-Col-SDF1α) were assessed and the mechanisms were explored. Furthermore, we investigated the cell differentiation-inducing potential of these conjugated nanoparticles on MSCs toward endothelial cells, neurons, osteoblasts and adipocytes. We then ultimately explored the process of cell entry and transportation of the nanoparticles. Using a mouse animal model and retro-orbital sinus injection, we traced in vivo biodistribution to determine the biosafety of the Au-Col-SDF1α nanoparticles. In summary, our results indicate that Au-Col is a promising drug delivery system; it can be used to carry SDF1α to improve MSC therapeutic efficiency.
- Subjects
MESENCHYMAL stem cell differentiation; DRUG delivery systems; CORE materials; REACTIVE oxygen species; MESENCHYMAL stem cells; SCANNING electron microscopy
- Publication
Cells (2073-4409), 2023, Vol 12, Issue 14, p1893
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells12141893