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- Title
In vitro susceptibility testing of Malassezia pachydermatis to gentamicin.
- Authors
Silva, Freddy A.; Ferrer, Otilia; Déniz, Soraya; Rosario, Inmaculada; Conde‐Felipe, Magnolia; Díaz, Esther L.; Acosta‐Hernández, Begoña
- Abstract
Background Two studies have observed that growth media containing gentamicin can inhibit the growth of the yeast organism Malassezia pachydermatis. The minimum inhibitory concentration ( MIC) of this bactericidal antibiotic for this organism has not been previously determined. Objective To evaluate the susceptibility of M. pachydermatis isolates to gentamicin. Methods The MIC of gentamicin was determined using a modified version of the M27-A3 microdilution method following the guidelines of the Clinical and Laboratory Standards Institute. A modified Christensen's urea broth was used to enhance the growth of the M. pachydermatis isolates. Visual and spectrophotometric end-point readings were performed to detect the presence or absence of yeast growth. Results The MIC50 and MIC90 of gentamicin were 8.12 μg/mL and 32.5 μg/mL, respectively; M. pachydermatis strains were classified as susceptible (S), intermediate (I) and resistant (R). The susceptibility of these isolates to gentamicin in vitro, by visual and spectrophotometric end-point reading, was: S, 54-56%; I, 40-41%; and R, 3-6%. Conclusion Prospective MICs for M. pachydermatis have been established for gentamicin.
- Subjects
MALASSEZIA; CRYPTOCOCCACEAE; GENTAMICIN; AMINOGLYCOSIDES; ANTIBACTERIAL agents
- Publication
Veterinary Dermatology, 2017, Vol 28, Issue 4, p348
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/vde.12427