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- Title
Integrin β1 Increases Stem Cell Survival and Cardiac Function after Myocardial Infarction.
- Authors
Lili Li; Qifan Guan; Shuling Dai; Wen Wei; Yao Zhang
- Abstract
Bone mesenchymal stem cells (BMSCs) transplantation is a promising therapeutic approach for myocardial infarction (MI), but its application is limited by poor viability of BMSCs. In this study, we aimed to improve the survival of BMSCs by lentivirus vector mediated overexpression of integrin β1. In vitro study showed that integrin β1 overexpression could facilitate the proliferation of BMSCs under oxygen glucose deprivation condition and regulated the expression of Caspase-3, Bax, Bcl-2, FAK, and ILK in BMSCs. Next, MI was induced in rat model and Igtβ1BMSCs, NullBMSCs, or NatBMSCs were transplanted by intramyocardial injection. One week later, the survival of BMSCs was higher in Itgβ1 BMSCs group than in other groups. Four weeks after transplantation, heart function was significantly improved in Igtβ1BMSCs group compared to other groups. The expression levels of Caspase-3 and Bax were decreased while the expression levels of Bcl-2, FAK, ILK, and VEGF were increased in the cardiomyocytes of Igtβ1BMSCs group compared to other groups. In conclusion, integrin β1 overexpression could increase the survival of BMSCs and improve the efficacy of transplanted BMSCs for MI treatment. The beneficial effects may be mediated by inhibiting the apoptosis of both transplanted BMSCs and cardiomyocytes through adhesion-mediated cell survival signaling.
- Subjects
STEM cells; HEART function tests; MYOCARDIAL infarction
- Publication
Frontiers in Pharmacology, 2017, Vol 8, p1
- ISSN
1663-9812
- Publication type
Article
- DOI
10.3389/fphar.2017.00135