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- Title
versatile strategy for convenient circular bivalent functional nucleic acids construction.
- Authors
Zhang, Xiao-Jing; Zhao, Zhuo; Wang, Xia; Su, Min-Hui; Ai, Lili; Li, Yingying; Yuan, Quan; Wang, Xue-Qiang; Tan, Weihong
- Abstract
Functional nucleic acids (FNAs), such as aptamers, nucleic acid enzymes and riboswitches play essential roles in various fields of life sciences. Tailoring of ingenious chemical moieties toward FNAs can enhance their biomedical properties and/or confer them with exogenic biological functions that, in turn, can considerably expand their biomedical applications, or even improve their clinical translations. Herein, we report the first example of a general chemical tailoring strategy that enables the divergent ligation of DNA sequences. By applying this technology, different types of aptamers and single-stranded nucleic acids of various lengths could be efficiently tailored to deliver the designed circular bivalent aptamers (CBApts) and cyclized DNA sequences with high yields. It is worth noting that CBApts exhibited significantly enhanced nuclease resistance, as well as considerably improved binding, targeting and tumor tissue enrichment abilities, which may pave the way for different investigations for biomedical purposes.
- Subjects
MOIETIES (Chemistry); APTAMERS; DNA sequencing; LIFE sciences; NUCLEIC acids; RIBOSWITCHES; CLICK chemistry
- Publication
National Science Review, 2023, Vol 10, Issue 2, p1
- ISSN
2095-5138
- Publication type
Article
- DOI
10.1093/nsr/nwac107