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- Title
NO signalling in cytokinin-induced programmed cell death.
- Authors
Carimi, Francesco; Zottini, Michela; Costa, Alex; Cattelan, Irene; De Michele, Roberto; Terzi, Mario; Lo Schiavo, Fiorella
- Abstract
Cell death can be induced by cytokinin 6-benzylaminopurine (BA) at high dosage in suspension-cultured Arabidopsis cells. Herein, we provide evidence that BA induces nitric oxide (NO) synthesis in a dose-dependent manner. A reduction in cell death can be observed when the cytokinin is supplemented with the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) or the nitric oxide synthase (NOS) inhibitors: 2-aminoethyl-isothiourea (AET) and N G.-monomethyl- l-arginine ( l-NMMA), which suggests that NO is produced via a NOS and is a signalling component of this form of programmed cell death. In BA-treated cells, mitochondrial functionality is altered via inhibition of respiration. This inhibition can be prevented by addition of either cPTIO or AET implying that NO acts at the mitochondrial level.
- Subjects
ARABIDOPSIS; BRASSICACEAE; CELL respiration; CYTOKININS; CELL death; PLANT cells &; tissues; MITOCHONDRIA
- Publication
Plant, Cell & Environment, 2005, Vol 28, Issue 9, p1171
- ISSN
0140-7791
- Publication type
Article
- DOI
10.1111/j.1365-3040.2005.01355.x