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- Title
Extracellular matrix surface network is associated with non-morphogenic calli of Helianthus tuberosus cv. Albik produced from various explants.
- Authors
Pilarska, Maria; Popielarska-Konieczna, Marzena; Ślesak, Halina; Kozieradzka-Kiszkurno, Małgorzata; Góralski, Grzegorz; Konieczny, Robert; Bohdanowicz, Jerzy; Kuta, Elżbieta
- Abstract
Helianthus tuberosus is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (i) the morphogenic capacity of callus in topinambur is genotype dependent, (ii) cv. Albik of H. tuberosus proved recalcitrant in in vitro regeneration, and (iii) extracellular matrix surface network is not a morphogenic marker in this cultivar.
- Subjects
JERUSALEM artichoke; EXTRACELLULAR matrix; SUNFLOWERS; CALLUS (Botany); REGENERATION (Botany)
- Publication
Acta Societatis Botanicorum Poloniae, 2014, Vol 83, Issue 2, p67
- ISSN
0001-6977
- Publication type
Article
- DOI
10.5586/asbp.2014.009