We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effect of CoCl<sub>2</sub> on the content of different metals and a relative activity of DNA-hydrolyzing abzymes in the blood plasma of mice.
- Authors
Legostaeva, Galina A.; Zaksas, Nataliya P.; Gluhcheva, Yordanka G.; Sedykh, Sergey E.; Madzharova, Maria E.; Atanassova, Nina N.; Buneva, Valentina N.; Nevinsky, Georgy A.
- Abstract
Cobalt is a transition metal and an essential trace element that is required for vitamin B12 biosynthesis, enzyme activation, and so on but is toxic in high concentrations. It was shown that the content of different elements in the plasma of 2-month-old BALB/c mice (control group) decreased in the following order: Ca > Mg > Si > Fe > Zn > Cu ≥ Al ≥ B. The treatment of mice with CoCl2 did not appreciably change the relative content of Ca, Cu, and Zn, but a significant increase in the content of B (2.3-fold), Mg (1.5-fold), Al and Fe (2.1-fold), and Si (3.4-fold) was found. The treatment of mice led to a 2.2-fold decrease in the concentration of the total blood protein and a 1.7 ± 0.2-fold decrease of total immunoglobulin Gs (IgGs). Deoxyribonuclease IgGs corresponding to mice treated (t-IgGs) and non-treated (nt-IgGs) with CoCl2 contained intrinsically bound metal ions; these IgGs hydrolyzed DNA with very low activity but were not active in the presence of ethylenediaminetetraacetic acid or after Ab dialysis against ethylenediaminetetraacetic acid. The average RAs of deoxyribonuclease nt-IgGs increased after addition of external metal ions in the following order: Zn2+ < Ca2+ < Cu2+ < Fe2+ < Mn2+ < Mg2+ < Co2+ < Ni2+. Interestingly, t-IgGs demonstrated lower activities than those for nt-IgGs either in the absence of external metal ions (2.7-fold) or in the presence of Cu2+ (9.5-fold) > Co2+ (5.6-fold) > Zn2+ (5.1-fold) > Mg2+ (4.1-fold) > Ca2+ (3.0-fold) > Fe2+ (1.3-fold). However, the RAs of t-IgGs were remarkably more active than nt-IgGs in the presence of best activators of t-IgGs Ni2+ (1.4-fold) and especially Mn2+ (2.2-fold). The data may be useful for an understanding of Co toxicity, its effect on the concentration of other metal ions, and a change of metal-dependent specificity of Abzs. Copyright © 2012 John Wiley & Sons, Ltd.
- Publication
Journal of Molecular Recognition, 2013, Vol 26, Issue 1, p10
- ISSN
0952-3499
- Publication type
Article
- DOI
10.1002/jmr.2217