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- Title
CpSmt3, an ortholog of small ubiquitin-like modifier, is essential for growth, organelle function, virulence, and antiviral defense in Cryphonectria parasitica.
- Authors
Shuangcai Li; Fengyue Chen; Xiangyu Wei; Luying Yuan; Jiayao Qin; Ru Li; Baoshan Chen
- Abstract
Introduction: SUMOylation is an important post-translational modification that regulates the expression, localization, and activity of substrate proteins, thereby participating in various important cellular processes such as the cell cycle, cell metabolism, gene transcription, and antiviral activity. However, the function of SUMOylation in phytopathogenic fungi has not yet been adequately explored. Methods: A comprehensive analysis composed of proteomics, affinity pulldown, molecular and cellular approaches was performed to explore the roles of SUMOylation in Cryphonectria parasitica, the fungal pathogen responsible for chestnut blight. Results and discussion: CpSmt3, the gene encoding the SUMO protein CpSmt3 in C. parasitica was identified and characterized. Deletion of the CpSmt3 gene resulted in defects in mycelial growth and hyphal morphology, suppression of sporulation, attenuation of virulence, weakening of stress tolerance, and elevated accumulation of hypovirus dsRNA. The 1CpSmt3 deletion mutant exhibited an increase in mitochondrial ROS, swollen mitochondria, excess autophagy, and thickened cell walls. About 500 putative SUMO substrate proteins were identified by affinity pull-down, among which many were implicated in the cell cycle, ribosome, translation, and virulence. Proteomics and SUMO substrate analyses further revealed that deletion of CpSmt3 reduced the accumulation of CpRho1, an important protein that is involved in TOR signal transduction. Silencing of CpRho1 resulted in a phenotype similar to that of 1CpSmt3, while overexpression of CpRho1 could partly rescue some of the prominent defects in 1CpSmt3. Together, these findings demonstrate that SUMOylation by CpSmt3 is vitally important and provide new insights into the SUMOylation-related regulatory mechanisms in C. parasitica.
- Subjects
PARASITIC wasps; POST-translational modification; SMALL ubiquitin-related modifier proteins; GENETIC translation; RIBOSOMES; PHYTOPATHOGENIC fungi; GENE expression; CELL cycle
- Publication
Frontiers in Microbiology, 2024, p1
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2024.1391855