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- Title
Molecular cloning and functional analysis of a Δ<sup>12</sup>-fatty acid desaturase from the Antarctic microalga Chlamydomonas sp. ICE-L.
- Authors
He, Yingying; Zheng, Zhou; An, Meiling; Chen, Hao; Qu, Changfeng; Liu, Fangming; Wang, Yibin; Miao, Jinlai; Hou, Xuguang
- Abstract
Chlamydomonas sp. ICE-L, which can thrive in extreme environments of the Antarctic, could represent a promising alternative for polyunsaturated fatty acid (PUFA) production. A new Δ12-fatty acid desaturase (FAD)-encoding gene (Δ12CiFAD), 1269 bp in size, was cloned from Chlamydomonas sp. ICE-L. Bioinformatics analysis showed that Δ12CiFAD-encoded protein was homologous to known FADs with conserved histidine motifs, and localized to the chloroplast. Functional analysis of Δ12CiFAD indicated that recombinant Synechococcus 6803 expressing Δ12CiFAD could accumulate C18:2, whereas recombinant Saccharomyces cerevisiae expressing this enzyme could not accumulate C18:2 or any other new fatty acids. These results indicate that Δ12CiFAD is a functional enzyme in the chloroplast that can adjust Chlamydomonas sp. ICE-L cell membrane fluidity to adapt to Antarctic extreme low-temperature environments, which give us insights into the frigostable and cold-resistant mechanisms of hypothermic organisms.
- Subjects
CHLAMYDOMONAS; FUNCTIONAL analysis; MOLECULAR cloning; ACID analysis; UNSATURATED fatty acids; FATTY acid desaturase; EXTREME environments
- Publication
3 Biotech, 2019, Vol 9, Issue 9, pN.PAG
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-019-1858-6