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- Title
Secretome Analysis of High- and Low-Virulent Bovine Pasteurella multocida Cultured in Different Media.
- Authors
Qiu, Yangyang; Wang, Jianan; He, Fang; Wu, Xiaoyan; Dan, Ruitong; Hardwidge, Philip R.; Li, Nengzhang; Peng, Yuanyi
- Abstract
Simple Summary: The absence of secretome information of P. multocida impedes the understanding of the mechanisms of infection and the development of subunit vaccines of P. multocida. In this study, we employed data-independent acquisition (DIA) LC-MS/MS combined with bioinformatics analysis to obtain more comprehensive and accurate information on proteins secreted by P. multocida. Then, the selected Tuf protein was verified by bioinformatics analysis, immunohistochemistry and immunoelectron microscopy. In addition, in this study, Tuf protein was expressed as recombinant antigen and tested as a subunit vaccine, and its biological characteristics were studied by serum antibody titer detection and Pasteurella survival assay. As a result, this study aims to offer a novel perspective and solid theoretical foundation for the investigation of the pathogenic mechanism of P. multocida and the design of vaccines. Bovine Pasteurella multocida (P. multocida) serotype A is one of the major causes of bovine respiratory disease (BRD). We used data-independent acquisition (DIA) LC-MS/MS combined with bioinformatics analysis to identify proteins secreted by P. multocida. A total of 154 proteins were obtained from the supernatants of two isolates of bovine P. multocida serotype A (high virulent PmCQ2 and low virulent PmCQ6) cultured in Martin or BHI media, of which 50 were identified as putative secreted proteins. Further studies showed that Tuf, an elongation factor Tu, was highly expressed in P. multocida and secreted into infected tissues. Tuf stimulated strong innate immune responses of macrophages and had protective efficacy against P. multocida infection in a mouse model. The results provide insight into the secreted proteins of P. multocida and suggest new targets for vaccine development against P. multocida.
- Subjects
PASTEURELLA multocida; IMMUNOGLOBULINS; IMMUNOELECTRON microscopy; BOS; ANTIBODY titer; VACCINE development; CELL culture
- Publication
Animals (2076-2615), 2023, Vol 13, Issue 23, p3683
- ISSN
2076-2615
- Publication type
Article
- DOI
10.3390/ani13233683