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- Title
Health-Promoting Potential of Mandarin Pomace Extracts Enriched with Phenolic Compounds.
- Authors
Fernández-Fernández, Adriana Maite; Dellacassa, Eduardo; Curbelo, Romina; Nardin, Tiziana; Larcher, Roberto; Medrano-Fernandez, Alejandra; del Castillo, María Dolores
- Abstract
The aim of this work was to assess the effect of in vitro human digestion on the chemical composition (carbohydrates and phenolic compounds) and bioactivity of hydro-alcoholic-acid pomace extracts from two mandarin varieties (Clemenule and Ortanique) by measuring their antioxidant, antidiabetic, anti-glycative, hypolipidemic, and anti-inflammatory properties. The phenolic compound profile showed that nobiletin was the main flavonoid found in the extracts and digests of Clemenule pomace and extract, while isosinensetin/sinensetin/tangeretin were the ones in the Ortanique samples. The digests of Clemenule and Ortanique extracts showed Folin reaction values of 9.74 and 9.20 mg gallic acid equivalents (GAE)/g of sample, ABTS values of 83.2 and 91.7 µmol Trolox equivalents (TE)/g of sample, and ORAC-FL values of 142.8 and 891.6 µmol TE/g of sample, respectively. Extracts (50–500 µg/mL) inhibited intracellular reactive oxygen species (ROS) formation in CCD-18Co cells under physiological and oxidative-induced conditions. Clemenule and Ortanique extract digests showed IC50 values of 13.50 and 11.07 mg/mL for α-glucosidase, 28.79 and 69.64 mg/mL for α-amylase, and 16.50 and 12.77 mg/mL for AGEs, and 2.259 ± 0.267 and 0.713 ± 0.065 mg/mL for pancreatic lipase inhibition, respectively. Ortanique extract (250–1000 µg/mL) inhibited the production of nitric oxide in RAW264.7 macrophages under inflammation-induced conditions, and intracellular ROS formation. In conclusion, altogether, the results supported the potential of mandarin extracts to be used as health promoters by reducing the risk of non-communicable chronic diseases.
- Subjects
IN vitro studies; ANTI-inflammatory agents; RESEARCH funding; ANTILIPEMIC agents; NITRIC oxide; MACROPHAGES; HYDROCARBONS; HYPOGLYCEMIC agents; PLANT extracts; REACTIVE oxygen species; DOSE-effect relationship in pharmacology; PHENOLS; DRUG efficacy; ANTIOXIDANTS; LIPASES; CARBOHYDRATE metabolism; BIOAVAILABILITY; AMYLASES; EVALUATION; PHARMACODYNAMICS
- Publication
Nutrients, 2024, Vol 16, Issue 14, p2370
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu16142370