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- Title
Combined Treatment of Mori folium and Mori Cortex Radicis Ameliorate Obesity in Mice via UCP-1 in Brown Adipocytes.
- Authors
Kim, Do-Sung; Lee, Hwa-Young; Kim, Hwa-Jin; Lee, Geum-Hwa; Lim, Young Jae; Ko, Bo Mi; Kim, Ji-Hyun; Kim, Tae Won; Kim, Hye Kyung; Kim, Tae Young; Hwang, Dae Il; Choi, Ha Kyoung; Ju, Seon Min; Chung, Myung Ja; Chae, Han-Jung
- Abstract
Mori Folium (Morus alba leaf, MF) and Mori Cortex Radicis (Morus alba root cortex, MR) have been studied for their anti-obesity effects by enhancing the browning process and inhibiting adipogenesis. However, important aspects of their protective mechanisms have not been thoroughly investigated, which could aid in developing functional food. Thus, this study aims to determine the synergistic effects of MF and MR against obesity and its associated mechanisms. In an in vitro cell culture model of brown adipocytes, a 1:1 mixture of MF and MR showed a synergistic effect on the expression of brown adipocyte-specific genes, including Ucp-1, Ppargc1a, Cbp/p300-interacting transactivator (Cited), Prdm16, Tbx1, and Fgf21 compared with either MF- or MR-treated conditions. Moreover, they demonstrated the involvement of cAMP and Ca2+ in induction of brown adipocyte-specific genes. In an in vivo model using HFD-fed mice, MF/MR significantly inhibited weight gain, plasma cholesterol, LDL, TG content, fat mass, and adipocyte size. Furthermore, MF/MR inhibited morphological alteration and the expressions of fatty acid synthesis genes such as Srebp1 and Fasn in the white adipose tissue. Thermogenesis genes were recovered in the brown adipose tissue with MF/MR supplementation, indicating that MF/MR regulated adipocytic dysmetabolism where AMPK signaling is involved. In conclusion, these results suggested that MF/MR regulates brown and beige adipocyte processes, providing one of the preventive functional food/herbal medicines against obesity and its associated metabolic diseases.
- Subjects
PROTEIN metabolism; OBESITY; IN vitro studies; HERBAL medicine; COMBINATION drug therapy; ANIMAL experimentation; AMP-activated protein kinases; LOW density lipoproteins; PLANT roots; TREATMENT effectiveness; WEIGHT gain; CELLULAR signal transduction; LEAVES; FAT cells; RESEARCH funding; GENES; BODY temperature regulation; CHINESE medicine; MICE; ADIPOSE tissues; CHOLESTEROL; FATTY acids
- Publication
Nutrients, 2023, Vol 15, Issue 17, p3713
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu15173713