We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Intraamniotic Administration (Gallus gallus) of Genistein Alters Mineral Transport, Intestinal Morphology, and Gut Microbiota.
- Authors
Cheng, Jacquelyn; Kolba, Nikolai; Sisser, Philip; Turjeman, Sondra; Even, Carmel; Koren, Omry; Tako, Elad
- Abstract
Genistein is an isoflavone naturally present in numerous staple food crops, such as soybeans and chickpeas. This study utilized the Gallus gallus intraamniotic administration procedure to assess genistein administration effects on trace mineral status, brush border membrane (BBM) functionality, intestinal morphology, and intestinal microbiome in vivo. Eggs were divided into five groups with 1 mL injection of the following treatments: no-injection, DI H2O, 5% inulin, and 1.25% and 2.5% genistein (n = 8 per group). Upon hatch, blood, cecum, small intestine, and liver were collected for assessment of hemoglobin, intestinal microflora alterations, intestinal morphometric assessment, and mRNA gene expression of relevant iron and zinc transporter proteins, respectively. This study demonstrated that intraamniotic administration of 2.5% genistein increased villus surface area, number of acidic goblet cells, and hemoglobin. Additionally, genistein exposure downregulated duodenal cytochrome B (DcytB) and upregulated hepcidin expression. Further, genistein exposure positively altered the composition and function of the intestinal microbiota. Our results suggest a physiological role for genistein administration in improving mineral status, favorably altering BBM functionality and development, positively modulating the intestinal microbiome, as well as improving physiological status.
- Subjects
KRUSKAL-Wallis Test; BIOMARKERS; REVERSE transcriptase polymerase chain reaction; ANALYSIS of variance; POULTRY; GUT microbiome; ANIMAL experimentation; GENE expression; GENISTEIN; DESCRIPTIVE statistics; TRACE elements; MINERALS; INTESTINAL mucosa; DATA analysis software; FETUS
- Publication
Nutrients, 2022, Vol 14, Issue 17, p3473
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu14173473