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- Title
Lentiviral vector-mediated transduction of adult neural stem/progenitor cells isolated from the temporal tissues of epileptic patients.
- Authors
Abdolahi, Sara; Khodakaram-Tafti, Azizollah; Aligholi, Hadi; Ziaei, Saeid; Ghadiri, Maryam Khaleghi; Stummer, Walter; Gorji, Ali
- Abstract
Objective(s): Neural stem/progenitor cells (NS/PCs) hold a great potential for delivery of therapeutic agents into the injured regions of the brain. Efficient gene delivery using NS/PCs may correct a genetic defect, produce therapeutic proteins or neurotransmitters, and modulate enzyme activation. Here, we investigated the efficiency of a recombinant lentivirus vector expressing green fluorescent protein (GFP) for genetic engineering of human NS/PCs obtained during brain surgery on patients with medically intractable epilepsy. Materials and Methods: NS/PCs were isolated from human epileptic neocortical tissues. Three plasmids (pCDH, psPAX2, pMD2.G) were used to make the virus. To produce the recombinant viruses, vectors were transmitted simultaneously into HEk-293T cells. The lentiviral particles were then used to transduce human NS/PCs. Results: Our in vitro study revealed that lentivirus vector expressing GFP efficiently transduced about 80% of human NS/PCs. The expression of GFP was assessed as early as 3 days following exposure and remained persistent for at least 4 weeks. Conclusion: Lentiviral vectors can mediate stable, long-term expression of GFP in human NS/PCs obtained from epileptic neocortical tissues. This suggests lentiviral vectors as a potential useful tool in human NS/PCs-based gene therapy for neurological disorders, such as epilepsy.
- Subjects
PROGENITOR cells; GREEN fluorescent protein; ENZYME activation; GENETIC engineering; RECOMBINANT viruses
- Publication
Iranian Journal of Basic Medical Sciences, 2020, Vol 23, Issue 3, p354
- ISSN
2008-3866
- Publication type
Article
- DOI
10.22038/IJBMS.2019.42285.9983