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- Title
EVALUATION OF SALIVA URIC ACID LEVELS IN YOUNG ADULT INDIVIDUALS BY BIOSENSOR METHOD.
- Authors
Kökbaş, Umut; Şemsi, Rabia; Arslan, Burak; Dinçel, Aylin Sepici; Ergünol, Erdal; Kayrın, Levent
- Abstract
Objectives:In this study we aimed to use a new amperometric biosensor for the determination of uric acid in saliva samples. Besides the good working conditions of the electrode was determined and identified the factors affecting the study. Uric acid is the final product of purine catabolism and related to different disease such as gout, increased levels of serum samples can be detected. Materials-Methods: By the prepared biosensor the used BSA/gelatin has crosslinked the uricase enzyme on the graphite electrode to glutaraldehyde. During characterization studies, linearity, repeatability of the measurements and shelf life were carried out. For the validation and verification studies, the students of the Faculty of Dentistry of the Cyprus Health and Social Sciences University formed the control group of the young adult individuals (n=26). Uric acid values from saliva samples were measured by autoanalyzer working with chemiluminescence principle and compared biosensor results. Results: It was determined that limit of detection 6. 0x10-4 mM, and linear results are obtained between the ranges of 6. 0x10-4 - 5,0x10-3 mM for uric acid concentration. As for shelf life studies, at the end of 40th day of responses has been preserved by 82. 5%, repeatability of the measurement (n=15) standard deviation (S. S) = ±0. 1 and % coefficient of variation (V. K)=0. 2, respectively. When the autoanalyser results were compared statistically to biosensor results, 97% accuracy rate was obtained from the drawn ROC curve. Conclusions: Today, different colorimetric, chromatographic and spectrophotometric methods are used for determination of uric acid. However, biosensor-based methods are preferred because of being practical and inexpensive systems. The most important advantage of biosensors over other methods is that they are economical, practical and specific for the substances to be determined.
- Subjects
YOUNG adults; SALIVA; URIC acid; RECEIVER operating characteristic curves; GLUTARALDEHYDE; STANDARD deviations; DETECTION limit
- Publication
Turkish Journal of Biochemistry / Turk Biyokimya Dergisi, 2019, Vol 44, p45
- ISSN
0250-4685
- Publication type
Article