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- Title
Evaluation on two types of paramyosin vaccines for the control of Haemaphysalislongicornis infestations in rabbits.
- Authors
Wu, Pin-Xing; Cui, Xue-Jiao; Cao, Mi-Xue; Lv, Li-Hong; Dong, Hong-Meng; Xiao, Shu-Wen; Liu, Jing-Ze; Hu, Yong-Hong
- Abstract
Background: Haemaphysalislongicornis is an obligate hematophagous ectoparasite that transmits a variety of pathogens causing life-threatening diseases in humans and animals. Paramyosin (Pmy) is not only an invertebrate-specific myofibrillar protein but also an important immunomodulatory protein. Therefore, it is one of the ideal candidate antigens for vaccines. Methods: We conducted two vaccine trials to evaluate the protective efficacy of Pmy recombinant protein (rPmy) and peptide vaccine (KLH-LEE). Each rabbit was immunized with three doses of rPmy or KLH-LEE adjuvanted with Freund's complete/incomplete at 500 μg/dose at 2-week intervals before challenge with 40 female H.longicornis/rabbit. PBS plus adjuvant, Trx or KLH was used as control group. The antibodies of rabbits were detected by ELISA. Then, female ticks were fed on the rabbits until detachment. Results: ELISA results showed that both vaccines induced rabbits to produce antibodies. Compared with the Trx group, the engorgement weight, oviposition and hatchability of the rPmy group decreased by 8.87%, 26.83% and 38.86%, respectively. On the other hand, engorgement weight, oviposition and hatchability of female ticks in the KLH-LEE group correspondingly resulted in 27.03%, 53.15% and 38.40% reduction compared with that of the KLH group. Considering the cumulative effect of vaccination on the evaluated parameters, results showed 60.37% efficacy of the rPmy vaccine formulation and 70.86% efficacy in the KLH-LEE group. Conclusions: Pmy and particularly epitope LEE have potential for further development of an effective candidate vaccine to protect the host against tick infection. Graphic abstarct:
- Subjects
RABBITS; RECOMBINANT proteins; VACCINE trials; VACCINE effectiveness; VACCINES; IMMUNOGLOBULINS
- Publication
Parasites & Vectors, 2021, Vol 14, Issue 1, p1
- ISSN
1756-3305
- Publication type
Article
- DOI
10.1186/s13071-021-04812-4