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- Title
Real-time PCR as a diagnostic tool for evaluating the resistance of Chinese cabbage cultivars to Verticillium wilt.
- Authors
Yu, Shuancang; Su, Tongbing; Chen, Juan; Wang, Weihong; Zhang, Fenglan; Yu, Yangjun; Zhang, Deshuang; Zhao, Xiuyun; Lu, Guixiang
- Abstract
Verticillium wilt disease has become increasingly serious in many regions in China over the past few years. In this study, the widespread pathogen was identified as Verticillium longisporum ( V. longisporum) by mt-SSU rDNA and cytochrome b sequence analysis. By using the primer pair HW1-F/HW1-R, which was designed based on the V. longisporum-specific rDNA-ITS sequence, a real-time PCR assay was developed for rapid and specific detection of the pathogen. The real-time PCR assay enabled sensitive quantification of V. longisporum in artificial and field-infected seedlings. This study provides a method for large-scale screening of Verticillium wilt-resistant resources for Chinese cabbage breeding.
- Subjects
CABBAGE disease &; pest resistance; VERTICILLIUM wilt diseases; POLYMERASE chain reaction; CHINESE cabbage; RECOMBINANT DNA; CYTOCHROME b
- Publication
European Journal of Plant Pathology, 2015, Vol 143, Issue 3, p549
- ISSN
0929-1873
- Publication type
Article
- DOI
10.1007/s10658-015-0706-8