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- Title
Reference gene validation for qPCR in rat carotid body during postnatal development.
- Abstract
The article presents a study in which six commonly used reference genes, b-actin, RPII (RNA polymerase II), PPIA (peptidyl-proyl-isomerase A), TBP (TATA-box binding protein), GAPDH, and 18s rRNA, were evaluated in two age groups under three environmental oxygen conditions using the three most commonly used software programs, geNorm, NormFinder and BestKeeper in order to determine the most stable and suitable reference genes for the study of rat carotid body during development. The study suggests that it is important for the selection of the best reference gene or combination of reference genes for carotid body development studies to take into account the environmental conditions.
- Subjects
PUBLIC health research; NONCHROMAFFIN paraganglia; HEREDITY; CHEMORECEPTORS; CAROTID body; REGULATION of respiration; RNA polymerases; CARRIER proteins; MEDICAL research
- Publication
BMC Research Notes, 2011, Vol 4, Issue 1, p440
- ISSN
1756-0500
- Publication type
Article
- DOI
10.1186/1756-0500-4-440