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- Title
Identification of a novel gill-specific calpain from rainbow trout ( Oncorhynchus mykiss).
- Authors
Salem, M.; Rexroad, C. E.; Yao, J.
- Abstract
Calpains are calcium-dependent neutral proteases responsible for many cellular functions. The two forms of calpain ubiquitously expressed in mammalian tissues are known as μ-calpain and m-calpain. We report here the identification of a novel calpain that is similar to but distinct from the μ- and m-calpains in rainbow trout. The cDNA of the novel gene is 2623 bp in length with a single open reading frame. The predicted protein (676 amino acids) contains the conserved calpain characteristic domains that include: domain I (pro peptide), II (cysteine catalytic site), III (electrostatic switch), and IV (calmodulin-like) with five Ca2+-binding EF hands. Northern blot and RT-PCR analyses demonstrated that the novel calpain gene is predominantly expressed in rainbow trout gills. Comparison of the novel protein with the ubiquitously expressed calpains and several mammalian tissue-specific calpains revealed that the novel calpain is an orthologue of the mammalian digestive tract specific calpain (calpain 9).
- Subjects
CALPAIN; RAINBOW trout; CYPRINUS; CARP; PROTEOLYSIS
- Publication
Fish Physiology & Biochemistry, 2006, Vol 32, Issue 1, p1
- ISSN
0920-1742
- Publication type
Article
- DOI
10.1007/s10695-005-0560-2