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- Title
High rate of neonates colonized by methicillin-resistant Staphylococcus species in an Intensive Care Unit.
- Authors
Salgueiro, Vivian Carolina; Lima Seixas, Milena D'Angelo; Guimarães, Lorrayne Cardoso; de Carvalho Ferreira, Dennis; da Cunha, Denise Cotrim; Nouér, Simone Aranha; dos Santos, Kátia Regina Netto
- Abstract
Introduction: Staphylococcal colonization is a risk factor for healthcare-associated infections, which are frequent in Neonatal Intensive Care Units (NICU). This study analyzed microbiology, epidemiology and clinical aspects of Staphylococcus spp. colonizing neonates. Methodology: Nasal or periumbilical swabs were evaluated from 175 newborns admitted to a NICU of a Rio de Janeiro hospital from March to September 2009. Clinical data were obtained from the medical records. SCCmec typing and the mecA and Panton-Valentine Leukocidin (PVL) genes were detected by PCR. Clonal diversity was evaluated by pulsed-field gel electrophoresis. Results: Staphylococcus spp. isolates were detected in 98 (56%) neonates, 66.3% of them had birth weight ≤ 2500 g, 62.2% were preterm (< 37 weeks) and the mean length of hospitalization was 14.9 days. Among the 133 isolates identified, 48.1% were S. epidermidis, 23.3% S. haemolyticus and 13.5% S. aureus. Methicillin-resistant Staphylococcus isolate was detected in 77.6% of neonates. The methicillin-resistant S. aureus isolates carried the SCCmec type IV, while 94.6% of S. epidermidis and 85.7% of S. haemolyticus presented non-typeable cassettes. Among the S. aureus, 55.6% had PVL genes and the USA800 genotype was prevalent. Two genotypes of S. epidermidis and one of S. haemolyticus clustered 42.2% and 25.8% of the isolates, respectively. S haemolyticus colonization was associated with the use of parenteral nutrition and mechanical ventilation. Conclusion: High rate of neonates colonized by methicillin-resistant Staphylococcus species and the permanence of clones circulating in the NICU highlight the importance for continuous and preventive surveillance in this high-risk population.
- Subjects
INTENSIVE care units; NEWBORN infants; PULSED-field gel electrophoresis; STAPHYLOCOCCUS; BIRTH weight; HAND care &; hygiene
- Publication
Journal of Infection in Developing Countries, 2019, Vol 13, Issue 9, p810
- ISSN
2036-6590
- Publication type
Article
- DOI
10.3855/jidc.11241