We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Prevalence of Bovine Viral Leukosis in the municipality of Ibagué-Tolima and nearby regions.
- Authors
Cabezas Callejas, Laura L.; Ramírez Navarro, Viviana M.; Lopera Vásquez, Ricaurte; Rondón Barragán, Iang S.
- Abstract
Background: the Bovine Viral Leukosis Virus (BLV) is a member of the Retroviridae family in the Deltaretrovirus genus‚ and is responsible for causing Enzootic Bovine Leukosis‚ a chronic infectious disease of great significance in Colombia. However‚ BVL epidemiology in IbaguéTolima‚ and their surrounding regions‚ where cattle breeding is relevant‚ is still undetermined. Objective: to determine the prevalence (nested PCR) and identify risk factors associated with BVL in Ibagué-Tolima and surrounding regions. Methods: for a cross-sectional study‚ blood samples from adult cattle (> 2 years) from different production systems were randomly collected (199 samples). Genomic DNA was extracted by the phenol-chloroformisoamyl alcohol method and DNA quality was verified with biomolecule spectrophotometry and amplification of a reference gene (actb) by conventional PCR. A nested PCR with specific primers was used to amplify the viral env gene‚ for BLV detection. By survey‚ zootechnical and management aspects as age‚ body score‚ breed‚ production purpose‚ sudden deaths‚ palpation gloves re-use and reproductive management were collected‚ and they were used for risk factors analysis. The data were subjected to Odds ratio (OR)‚ Prevalence ratio (PR), and Chi-square (χ2) test‚ a p-value < 0.05 was considered significant. Results: actually‚ 99 out of 199 samples (49.75 %) tested positive for the BLV env gene. 3 tested positive (Sanger) samples were sequenced and aligned with Genbank reports (2 national and 2 international). Palpation gloves reuse 53.54 %; OR = 2.4484; PR = 1.545; χ2 = 9.4291‚ p = 0.0021, and sudden death 72.73 %; OR = 3.1304‚ PR = 1.831; χ2‚ p = 0.0001 were identified as risk factors. Conclusions: reuse of palpation gloves and sudden death on farms is associated with higher prevalence of BLV. The municipality of Ibagué present a high prevalence of the virus‚ it is important to extend the research to the departmental level and determine the associated risk factors to carry out effective control strategies.
- Subjects
COLOMBIA; SURGICAL gloves; BOVINE leukemia virus; LEUKEMIA; SUDDEN death; CATTLE breeds; BOS
- Publication
Revista Colombiana de Ciencias Pecuarias, 2024, Vol 37, p207
- ISSN
0120-0690
- Publication type
Article