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- Title
Gender Identification of Domesticated Chicken Using a PCR-based Method.
- Authors
Aun, Richard Teh Swee; Kumaran, Jayaraj Vijaya
- Abstract
The monomorphism of newly hatched chicks poses a problem for farmers who need to separate them. Currently, the most widely used technique is the cloacal sexing method which has a low accuracy rate. Thus, a PCR based gender determination protocol was established to obtain a higher accuracy rate than conventional sexing methods. Feathers of a day up to a week old chicks were collected for the molecular analysis using the PCR targeting the CHD- Wand CHD-Z genes in the chicken's chromosome Z and chromosome W, respectively. The results of this study showed that the PCR based gender determination protocol was a sensitive and accurate method for determining the gender of monomorphic chicks as compared to the conventional sexing method.
- Subjects
CHICKENS; DOMESTIC animal genetics; ANIMAL genetics; GENDER identity; POLYMERASE chain reaction; POLYMERIZATION; PHYSIOLOGY
- Publication
Pertanika Journal of Tropical Agricultural Science, 2010, Vol 33, Issue 2, p329
- ISSN
1511-3701
- Publication type
Article