We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Laboratory Reporting Accuracy of Polymerase Chain Reaction Testing for Avian Polyomavirus.
- Authors
Fitzgerald, Brenna; Olsen, Geoff; Speer, Brian
- Abstract
Polymerase chain reaction (PCR) assays are available for detection of birds infected with avian polyomavirus (APV). Several laboratories offer this diagnostic assay in the United States, but little information is available regarding assay sensitivity, specificity, and accuracy. In this study, known APV-positive and APV-negative samples (each n = 10, 5 undiluted and 5 diluted) were sent to 5 commercial laboratories. A significant difference in reporting accuracy was found among laboratories, most notably for dilute APV-positive samples. Two out of 5 laboratories provided 100% accurate results, 1 had an accuracy of 90%, and 2 reported 80% and 75% accuracy, respectively. The accuracies of the last 2 laboratories were negatively affected by test sensitivities of 60% and 50%, respectively. These findings show that although accurate results were reported by most laboratories, both false-positive and false-negative results were reported by at least 3 laboratories, and false-negative results reported for dilute APV-positive samples predominated. These study findings illustrate a need for veterinary diagnostic laboratories to institute improved voluntary quality control measures.
- Subjects
POLYMERASE chain reaction; BIRD disease diagnosis; POLYOMAVIRUSES; VETERINARY diagnostic imaging; VETERINARY diagnosis; ROUTINE diagnostic tests
- Publication
Journal of Avian Medicine & Surgery, 2013, Vol 27, Issue 1, p32
- ISSN
1082-6742
- Publication type
Article
- DOI
10.1647/2012-014