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- Title
Glycan optimization of a human monoclonal antibody in the aquatic plant Lemna minor.
- Authors
Cox, Kevin M.; Sterling, Jason D.; Regan, Jeffrey T.; Gasdaska, John R.; Frantz, Karen K.; Peele, Charles G.; Black, Amelia; Passmore, David; Moldovan-Loomis, Cristina; Srinivasan, Mohan; Cuison, Severino; Cardarelli, Pina M.; Dickey, Lynn F.
- Abstract
N-glycosylation is critical to the function of monoclonal antibodies (mAbs) and distinguishes various systems used for their production. We expressed human mAbs in the small aquatic plant Lemna minor, which offers several advantages for manufacturing therapeutic proteins free of zoonotic pathogens. Glycosylation of a mAb against human CD30 was optimized by co-expressing the heavy and light chains of the mAb with an RNA interference construct targeting expression of the endogenous α-1,3-fucosyltransferase and β-1,2-xylosyltransferase genes. The resultant mAbs contained a single major N-glycan species without detectable plant-specific N-glycans and had better antibody-dependent cell-mediated cytotoxicity and effector cell receptor binding activities than mAbs expressed in cultured Chinese hamster ovary (CHO) cells.
- Subjects
MONOCLONAL antibodies; LEMNA minor; GLYCOSYLATION; GENE expression; RNA-protein interactions; CELL-mediated cytotoxicity
- Publication
Nature Biotechnology, 2006, Vol 24, Issue 12, p1591
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt1260