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- Title
Marrow mesenchymal stem cells transduced with TPO/FL genes as support for ex vivo expansion of hematopoietic stem/progenitor cells.
- Authors
Xie, C.-G.; Wang, J.-F.; Xiang, Y.; Jia, B.-B.; Qiu, L.-Y.; Wang, L.-J.; Wang, G.-Z.; Huang, G.-P.
- Abstract
A new marrow-derived mesenchymal stem cell (hMSC) line that could support expansion of hematopoietic stem/progenitor cells (HSPCs) was developed. Primary hMSCs were infected with retrovirus containing Flt-3 ligand and thrombopoietin genes. CD34+ cells from cord blood were expanded with primary hMSCs or transduced hMSCs. The expansion of total nucleated cells, CD34+ cells and mixed colonies containing erythroid and myeloid cells and megakaryocytes for 2 weeks coculture with transduced hMSCs was remarkably increased. The outputs of long-term culture-initiating cells for 2 and 4 weeks coculture with transduced hMSCs were also largely increased. The expansion rates of HSPCs with transduced hMSCs were unchanged for 6 weeks. In contrast, the expansion rates of HSPCs with primary hMSCs declined drastically through 6 weeks. SCID-repopulating cell expansion with transduced hMSCs for 4 weeks was significantly higher than that of uncultured CD34+ cells and HSPCs expanded with primary hMSCs.
- Subjects
STEM cells; BONE marrow; HEMATOPOIETIC growth factors; THROMBOPOIETIN; HEREDITY; CORD blood; MEGAKARYOCYTES; BONE marrow cells
- Publication
Cellular & Molecular Life Sciences, 2005, Vol 62, Issue 21, p2495
- ISSN
1420-682X
- Publication type
Article
- DOI
10.1007/s00018-005-5274-1