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- Title
EFFECT OF ADDITION OF CHOLESTEROL LOADED CYCLODEXTRIN (CLC) ON SPERM ABNORMALITIES DURING CRYOPRESERVATION OF POITOU JACK SEMEN.
- Authors
Kumar, Pramod; Mehta, J. S.; Ravi, S. K.; Purohit, G. N.; Chaudhary, Ashok Kumar; Dedar, Ramesh Kumar; Talluri, T. R.
- Abstract
The current study was conducted to investigate the effect of addition of cholesterol loaded cyclodextrin (CLC) on sperm morphological abnormalities of cooled and frozen thawed semen of Poitou jacks. Six adult Poitou jacks aged between 4 to 7 years were used for the present study and eight ejaculates from each jack was collected and were processed immediately after semen collection and subjected to the gross semen quality analysis. The sperm morphologies of fresh semen were analyzed by staining with eosin and nigrosine staining. The semen sample of each Poitou jack was divided into five equal fractions which were incubated for 15 minutes in water bath at 37°C after addition of primary extender without CLC (C); and with 1, 1.5, 2 and 3 mg/ml CLC (T1, T2 T3 and T4, respectively) to obtain 120 x 106 sperm/ml spermatozoa concentration. All the 5 aliquots were cryopreserved. Each cooled and frozen thawed semen samples, after thawing at 37°C for 30 sec were analyzed for sperm morphologies. From the current studies it was observed that treatment of Poitou jack semen samples with CLC could not reduce any significant sperm abnormalities at either prefreeze and post thaw stage and this was also true for addition of different concentration of CLC.
- Subjects
SEMEN analysis; FROZEN semen; SEMEN; SPERMATOZOA; CHOLESTEROL; HUMAN abnormalities
- Publication
Veterinary Practitioner, 2019, Vol 20, Issue 2, p218
- ISSN
0972-4036
- Publication type
Article