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- Title
Phenotypic analysis of Dlx5 overexpression in post-natal bone.
- Authors
Zhang J; Zhu J; Valverde P; Li L; Pageau S; Tu Q; Nishimura R; Yoneda T; Yang P; Zheng W; Ma W; Chen J; Zhang, J; Zhu, J; Valverde, P; Li, L; Pageau, S; Tu, Q; Nishimura, R; Yoneda, T
- Abstract
Dlx5 plays an important role in the embryonic development of mineralized tissues. We hypothesized that Dlx5 also functions in regulating post-natal bone formation in mice. To prove this hypothesis, we infected 5-day-old bone sialoprotein (BSP)/avian retroviral receptor gene (TVA) transgenic mice with replication-competent retroviral vectors expressing wild-type Dlx5 (RCAS-Dlx5WT) and mutated Dlx5 at arginine (R) 31 of its homeodomain (RCAS-Dlx5RH). Immunohistochemistry indicated that RCAS-Dlx5WT increased BSP and osteopontin (OPN) expression, whereas it decreased that of osteocalcin (OC). RCAS-Dlx5RH mediated opposite effects. Semi-quantitative RT-PCR confirmed these results. Ex vivo overexpression of RCAS-Dlx5WT in BSP/TVA calvarial cells promoted, whereas that of RCAS-Dlx5RH inhibited, mineralized nodule formation as compared with that in control cells. Our results suggest that Dlx5 promotes expression of early markers of osteogenic differentiation and increases mineralization post-natally.
- Publication
Journal of Dental Research, 2008, Vol 87, Issue 1, p45
- ISSN
0022-0345
- Publication type
journal article
- DOI
10.1177/154405910808700107