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- Title
In vitro cytotoxicity of resin cement and its influence on the expression of antioxidant genes.
- Authors
Moralez, Priscila F. A.; Kantovitz, Kamila R.; Martinez, Elizabeth F.; Teixeira, Lucas N.; Demasi, Ana P. D.
- Abstract
Aim: This study evaluated cytotoxicity and antioxidant gene expression of resin cements on human gingival fibroblasts (hGF). Materials and Method: RelyX Ultimate™(RXU), Variolink™ II(VLII), and RelyXU200™(RXU200) resin cements were incubated with culture medium for 24 h to obtain eluates. Then, the eluates were applied over hGF to assess cell viability at 24 h, 48 h, and 72 h and antioxidant gene expression at 24 h. hGF cultures non-exposed to the eluates were used as Control. Data were submitted to ANOVA and Bonferroni tests (α≤0.05). Results: RXU and RXU200 reduced the number of viable cells in 24 h. Longer exposure to cement extracts caused cell death. Gene expression showed peroxiredoxin 1 (PRDX1) induction by all resin cement types, and superoxide dismutase 1 (SOD1) induction by RXU200 and VLII. Moreover, RXU200 induced not only PRDX1 and SOD1, but also glutathione peroxidase 1 (GPX1), catalase (CAT), and glutathione synthetase (GSS). Conclusions: All resin cements showed toxicity, and induced antioxidant genes in hGF. Antioxidant gene induction is at least partly associated with cytotoxicity of tested cements to oxidative stress experience.
- Subjects
CYTOTOXINS; GENE expression; GLUTATHIONE peroxidase; SUPEROXIDE dismutase; OXIDATIVE stress; CELL death
- Publication
Acta Odontologica Latinoamericana: AOL, 2023, Vol 36, Issue 2, p120
- ISSN
0326-4815
- Publication type
Article
- DOI
10.54589/aol.36/2/120