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- Title
Nephro-Protective Effects of Origanum Vulgare HydroAlcoholic Extract against Gentamicin-Induced Nephrotoxicity in Rats.
- Authors
Mirzaei, Ali; Maleki, Reza; Ghavamizadeh, Mehdi
- Abstract
Background: Origanum Vulgare has been used in Iranian folk medicine to treat diaphoretic, carminative, antispasmodic, antiseptic and inflammatory disorders. Objectives: In this experimental study the nephroprotective effect of hydro-alcoholic extract of Origanum vulgare (HEOV) against Gentamicin (GM)-induced renal toxicity in rats was investigated. Materials and methods: The rats (n = 30) were randomly allocated to five groups of six rats each: group I received distilled water (DW) (p.o), group II received (GM 80 mg/kg) (i.p), group III received (GM 80 mg/kg + HEOV 100 mg/kg p.o), group IV received (GM 80 mg/kg + HEOV 250 mg/kg) and group V received (GM 80 mg/kg + HEOV 500 mg/kg). Nephrotoxicity was induced by intraperitoneal injection of Gentamicin (80 mg/kg) for 12 days. At the end of period rats were euthanized by ether anesthesia. Blood was collected and kidneys removed for biochemical and histopathological examinations respectively. Serum level of malon dialdchyde (MDA) was measured as markers of nephrotoxicity. Antioxidant activity and Nitric Oxide (NO) radical scavenging of HEOV were also assayed by in vitro method. Results: HEOV showed a potent antioxidant and NO radical scavenging activity by in vitro method. Administration of gentamicin to rats induced a marked renal failure, demonstrated with significant increase in serum level of Cr, BUN and MDA. Orally administration of HEOV at dose 100 mg/kg significantly (p < 0.01) normalized the alteration in above parameters. These findings were confirmed by histopathological examinations. Conclusions: use of HEOV in low dose could ameliorate Gentamicin-induced renal toxicity in rats probably through its radical scavenging activity.
- Subjects
NEPHROTOXICOLOGY -- Risk factors; ANIMAL experimentation; ANTIOXIDANTS; BIOMARKERS; COMBINATION drug therapy; GENTAMICIN; HYDROCARBONS; MEDICINAL plants; HEALTH outcome assessment; RATS; STATISTICAL sampling; WATER; MALONDIALDEHYDE; PLANT extracts; FREE radical scavengers; IN vitro studies
- Publication
International Medical Journal, 2016, Vol 23, Issue 1, p26
- ISSN
1341-2051
- Publication type
Article