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- Title
Study of the antineoplastic effect of modulating apoptosisrelated noncoding RNA in human hepatocellular carcinoma cell line (HepG2).
- Authors
Azeem Sherif, Mai Abdel; Ismail, Emtiaz Abd-elkawy; Kassim, Samar Kamal; Shehata, Hanan Hussein; Abdel Khalek, Marwa Ali; Abdel Hamid, Marwa Mahmoud
- Abstract
MiR-421 is considered an important molecule that can prevent tumor growth. Bioinformatics analysis indicated that mRNA caspase-3 gene is a target gene of miR-421. The current study aimed to explore the functional role of miR-421 in hepatocellular carcinoma (HCC) and explore the interaction between miR- 421 and caspase-3. To validate bioinformatics data, RT-qPCR was used to detect the expression of miR-421 and caspase-3 in 10 HCC tissues. The results showed miR-421 expression was significantly higher in HCC than non HCC liver tissues (P<0.01), nevertheless caspase-3 gene expression was markedly lower in HCC than non HCC liver tissues (P<0.01). Besides, miR-421 expression was negatively associated with caspase-3 expression. MiR-421 mimic and inhibitor was transfected into HCC cell lines (HepG2). Proliferation assay, showed that low-expression of miR-421 inhibited the proliferation of HCC cells. RT-qPCR was worked for detection the expression levels of miR-421 and caspase- 3 in HepG2 cells before and after transfection. The results showed that miR-421 expression in HepG2 cells was significantly lower in miR-421 inhibitor transfected group than in mimic- transfected and control groups (Mock) (P ≤ 0.05), and caspase- 3 gene expression in HCC tissues was markedly higher in inhibitor transfected group than those transfected by mimic and control group (Mock) (P ≤ 0.05). Thus, miR-421 inhibitor may inhibit the proliferation of HCC cells via over- expression of caspase- 3.
- Subjects
NON-coding RNA; HEPATOCELLULAR carcinoma; INHIBITION of cellular proliferation; CELL lines; CASPASES
- Publication
QJM: An International Journal of Medicine, 2021, Vol 114, pi53
- ISSN
1460-2725
- Publication type
Article
- DOI
10.1093/qjmed/hcab088.008