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- Title
Fine mapping of the Pc locus of Sorghum bicolor, a gene controlling the reaction to a fungal pathogen and its host-selective toxin.
- Authors
Nagy, Ervin D.; Lee, Tso-Ching; Ramakrishna, Wusirika; Xu, Zijun; Klein, Patricia E.; SanMiguel, Phillip; Cheng, Chiu-Ping; Li, Jingling; Devos, Katrien M.; Schertz, Keith; Dunkle, Larry; Bennetzen, Jeffrey L.
- Abstract
Milo disease in sorghum is caused by isolates of the soil-borne fungus Periconia circinata that produce PC-toxin. Susceptibility to milo disease is conditioned by a single, semi-dominant gene, termed Pc. The susceptible allele ( Pc) converts to a resistant form ( pc) spontaneously at a gametic frequency of 10−3 to 10−4. A high-density genetic map was constructed around the Pc locus using DNA markers, allowing the Pc gene to be delimited to a 0.9 cM region on the short arm of sorghum chromosome 9. Physically, the Pc-region was covered by a single BAC clone. Sequence analysis of this BAC revealed twelve gene candidates. Several of the predicted genes in the region are homologous to disease resistance loci, including one NBS-LRR resistance gene analogue that is present in multiple tandem copies. Analysis of pc isolines derived from Pc/Pc sorghum suggests that one or more members of this NBS-LRR gene family are the Pc genes that condition susceptibility.
- Subjects
GENE mapping; SORGHUM; HOST-fungus relationships; SOILBORNE plant pathogens; MICROBIAL toxins; PHYTOPATHOGENIC microorganisms; MILO; GENETIC markers
- Publication
Theoretical & Applied Genetics, 2007, Vol 114, Issue 6, p961
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-006-0481-1