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- Title
Antiproliferative activity of extracts of Euphorbia tirucalli L (Euphorbiaceae) from three regions of Brazil.
- Authors
do C. Caxito, Marina L.; Victório, Cristiane P.; da Costa, Helber B.; Romão, Wanderson; Kuster, Ricardo M.; Gattass, Cerli R.
- Abstract
Purpose: To investigate Euphorbia tirucalli extract for probable geographic variations in its antiproliferative activity. Methods: The aerial parts of E. tirucalli were collected in the Brazilian states of Mato Grosso, Rio de Janeiro, Pará, Minas Gerais and Santa Catarina. The 70 % ethanol extract was obtained according to the procedure described in Brazilian Homeopathic Pharmacopeia. The antiproliferative activity of extracts, in concentrations of 62, 125, 250, and 500 μg mL-1, was tested against leukemia (HL-60), lymphoma (Daudi) and melanoma (B16F10) cell lines using methyl thiazol tetrazolium assay (MTT). Phytochemical analysis were carried out using High-performance liquid chromatography-diode array (HPLC-UV-DAD) and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI(-) FT-ICR MS) assays. Results: There was significant regional variability in the cytotoxicity of E. tirucalli extracts in a dosedependent manner. The extracts had similar activity towards leukemia cell line HL-60, decreasing cell viability to about 60 - 70 %. The extract showed the presence of ellagitannins, flavonoids, veracylglucan, and acid triterpenes as the major compounds. Conclusion: While the results support the ethnopharmacological use of E. tirucalli throughout Brazil, regional quantitative differences found in some classes of secondary metabolites may explain the variations observed in antitumor activity.
- Subjects
BRAZIL; ANTINEOPLASTIC agents; EUPHORBIA; PLANT extracts; LEUKEMIA treatment; HIGH performance liquid chromatography; ELECTROSPRAY ionization mass spectrometry
- Publication
Tropical Journal of Pharmaceutical Research, 2017, Vol 16, Issue 5, p1013
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v16i5.7