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- Title
Antihypertensive Activity of Aqueous-Methanol Extract of Berberis Orthobotrys Bien Ex Aitch in Rats.
- Authors
Akhtar, Muhamamd S.; Jabeen, Qaiser; Akram, Muhammad; Khan, Hafeez U.; Karim, Sabeha; Malik, Muhammad N. H.; Mushtaq, Muhammad N.; Salma, Umme
- Abstract
Purpose: To investigate the hypotensive potential of Berberis orthobotrys Bien Ex Aitch (Family: Berberidaceae) in both normotensive and hypertensive rats. Methods: Aqueous-methanol (70:30) extract of Berberis orthobotrys at doses of 25, 50, 75 and 100 mg/kg was evaluated for its effect on blood pressure and heart rate using non-invasive blood pressure measuring apparatus. After initial screening, 100 mg/kg dose that produced a maximum effect was selected for the antihypertensive study. Median lethal dose (LD50) and sub-chronic toxicity of the extract were also determined. Various biochemical parameters and organ weight were measured using standard procedures. Results: The extract produced a significant (p < 0.01) decrease in systolic blood pressure (SBP), mean blood pressure (MBP), diastolic blood pressure (DBP) and heart rate of normotensive rats at all test doses with maximum effect at 100 mg/kg. Similarly, a significant antihypertensive and negative chronotropic effect was observed in both hypertensive models. LD50 of the extract was 200 mg/kg in mice. The extract also exhibited a reduction (p < 0.05) in serum alanine transaminase (ALT), aspartate aminotransaminase (AST), alkaline phosphatase (ALP), triglycerides and low density lipoprotein (LDL) levels while a significant (p < 0.05) increase in high density lipoproteins (HDL) level was observed. Conclusion: It seems that the aqueous-methanol extract of Berberis orthobotrys possesses active compounds which may be responsible for the antihypertensive and negative chronotropic effects in rats.
- Subjects
BERBERIDACEAE; ANTIHYPERTENSIVE agents; DRUG activation; AQUEOUS solutions; PLANT extracts; LABORATORY rats; PHARMACODYNAMICS
- Publication
Tropical Journal of Pharmaceutical Research, 2013, Vol 12, Issue 3, p393
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v12i3.18