We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Leishmania spp. PARASITE ISOLATION THROUGH INOCULATION OF PATIENT BIOPSY MACERATES IN INTERFERON GAMMA KNOCKOUT MICE.
- Authors
de Oliveira, Milton Adriano Pelli; da Silva Pires, Alause; de Bastos, Rosidete Pereira; de Araujo Lima, Glória Maria Collet; Pinto, Sebastião Alves; de Araujo Pereira, Ledice Inácia; Pereira, Ana Joaquina Cohen Serique; de Almeida Abrahamsohn, Ises; Dorta, Miriam Leandro; Ribeiro-Dias, Fátima
- Abstract
Isolation of Leishmania parasite and species identification are important for confirmation and to help define the epidemiology of the leishmaniasis. Mice are often used to isolate pathogens, but the most common mouse strains are resistant to infection with parasites from the Leishmania (Viannia) subgenus. In this study we tested the inoculation of interferon gamma knockout (IFNγ KO) mice with biopsy macerates from Leishmania-infected patients to increase the possibility of isolating parasites. Biopsies from twenty five patients with clinical signs of leishmaniasis were taken and tested for the presence of parasites. Immunohistochemical assay (IHC) and conventional histopathology detected the parasite in 88% and 83% of the patients, respectively. Leishmania sp. were isolated in biopsy macerates from 52% of the patients by culture in Grace's insect medium, but 13% of isolates were lost due to contamination. Inoculation of macerates in IFNγ KO mice provides isolation of parasites in 31.8% of the biopsies. Most isolates belong to L. (Viannia) subgenus, as confirmed by PCR, except one that belongs to L. (Leishmania) subgenus. Our preliminary results support the use of IFNγ KO mice to improve the possibility to isolate New World Leishmania species.
- Subjects
BRAZIL; LEISHMANIA; LEISHMANIASIS; BIOPSY; INJECTIONS; INTERFERONS; EPIDEMIOLOGY; LABORATORY mice; PATHOGENIC microorganisms; HISTOPATHOLOGY
- Publication
Revista do Instituto de Medicina Tropical de São Paulo, 2010, Vol 52, Issue 2, p83
- ISSN
0036-4665
- Publication type
Article
- DOI
10.1590/S0036-46652010000200004