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- Title
A Microfluidic System for Saliva-Based Detection of Infectious Diseases.
- Authors
CHEN, ZONGYUAN; MAUK, MICHAEL G.; WANG, JING; ABRAMS, WILLIAM R.; CORSTJENS, PAUL L. A. M.; NIEDBALA, R. SAM; MALAMUD, DANIEL; BAU, HAIM H.
- Abstract
A “lab-on-a-chip” system for detecting bacterial pathogens in oral fluid samples is described. The system comprises: (1) an oral fluid sample collector; (2) a disposable, plastic microfluidic cassette (“chip”) for sample processing including immunochromatographic assay with a nitrocellulose lateral flow strip; (3) a platform that controls the cassette operation by providing metered quantities of reagents, temperature regulation, valve actuation; and (4) a laser scanner to interrogate the lateral flow strip. The microfluidic chip hosts a fluidic network for cell lysis, nucleic acid extraction and isolation, PCR, and labeling of the PCR product with bioconjugated, upconverting phosphor particles for detection on the lateral flow strip.
- Subjects
MEDICAL technology; MICROFLUIDICS; PATHOGENIC microorganisms; COMMUNICABLE diseases; DIAGNOSIS; CHROMATOGRAPHIC analysis
- Publication
Annals of the New York Academy of Sciences, 2007, Vol 1098, p429
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1196/annals.1384.024