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- Title
Copper-induced oxidative damage, antioxidant response and genotoxicity in Lycopersicum esculentum Mill. and Cucumis sativus L.
- Authors
İşeri, Özlem; Körpe, Didem; Yurtcu, Erkan; Sahin, Feride; Haberal, Mehmet
- Abstract
Adequate copper (Cu) concentrations are required for plants; however, at higher concentrations it can also cause multiple toxic effects. In the present study, lipid peroxidation, hydrogen peroxide levels as well as ascorbate peroxidase (APX: EC 1/11/1/11) and catalase (CAT: EC 1.11.1.6) activities were determined in Lycopersicum esculentum Mill. and Cucumis sativus L. seedlings after 7-day exposure to copper sulfate. In addition, DNA damage in these two crops was assessed by measuring micronucleus (MN) frequency and tail moments (TM) as determined by Comet assay. Inhibitory copper concentrations (EC: 30 and 5.5 ppm for L. esculentum and C. sativus, respectively) were determined according to dose-dependent root inhibition curves, and EC and 2×EC were applied. Malondialdehyde (MDA) and HO levels significantly increased in all groups studied. CAT activity increased in treatment groups of C. sativus. APX activity increased in L. esculentum seedlings due to 2×EC treatment. Reductions in mitotic indices (MI) represented Cudependent root growth inhibition in all treatment groups studied. According to TMs and MN frequencies, copper exposure induced significant DNA damage ( p < 0.05) in all study groups, whereas the DNA damage induced was dose dependent in C. sativus roots. In conclusion, Cuinduced oxidative damage, elevations in HO levels and alterations in APX and CAT activities, as well as significant DNA damage in nuclei of both study groups. To our knowledge, this is the first comparative and comprehensive study demonstrating the effects of copper on two different plant species at relevant cytotoxic concentrations at both biochemical and genotoxicity levels with multiple end points.
- Subjects
COPPER; DNA damage; ANTIOXIDANTS; GENETIC toxicology; TOMATOES; CUCUMBERS
- Publication
Plant Cell Reports, 2011, Vol 30, Issue 9, p1713
- ISSN
0721-7714
- Publication type
Article
- DOI
10.1007/s00299-011-1079-x